Fighting a citrus killer – from the University of California Posted on February 24, 2017 Share on Facebook Share on Twitter Share on Google+ Share on Reddit Share on Pinterest Share on Linkedin Share on Tumblr Microbes cultured from the leaf microbiota of a Valencia orange tree. The researchers are working towards harnessing the chemistry that these microbes produce to develop novel antimicrobials against the causal agent of Huanglongbing. Credit: UC Riverside – UC Riverside researchers (from left) Philippe Rolshausen, David Jassby, Haizhou Liu, Caroline Roper, Georgios Vidalakis and James Borneman received a $5.1 million grant to fight a disease killing citrus trees. Riverside, Calif., (February 24, 2017) – A team of scientists, led by a group at the University of California, Riverside, has received a five-year, $5.1 million grant from the U.S. Department of Agriculture to fight a disease that is devastating the citrus industry. The team, led by Caroline Roper, an associate professor of plant pathology, will design and identify bactericides, which are chemicals that kill bacteria, to target Huanglongbing, a bacterial plant disease decimating citrus trees worldwide. They also will focus on better understanding the pathways those bactericides travel inside citrus trees. Huanglongbing, which has devastated citrus trees in Asia and South America, was detected in Florida in 2005 and has since led to a 75 percent decline in the Florida’s $9 billion citrus industry. Fifteen U.S. States or territories are under full or partial quarantine due to the presence of the Asian citrus psyllid, an insect which feeds on citrus trees and – in doing so – transmits Huanglongbing. Past research has identified the bacterium (Candidatus Liberibacter asiaticus or CLas) associated with Huanglongbing that is killing citrus trees. But, it has proved difficult to deliver bactericides to the phloem, the part of the citrus tree where the harmful bacteria resides. The UC Riverside team will analyze phloem transit routes that bactericides take when introduced through common application methods, such as trunk injection or leaf or root applications. They also will continue to develop a new delivery system for use in field citrus trees. The delivery system targets the branches and petioles, which are the stalk that join a leaf to a stem. The idea is based on previous work that indicates that this is an effective and efficient way to tap into and introduce material into phloem tissue, a tissue that is hard to access. They also will conduct fluorescent tracer experiments that map phloem transport pathways in citrus and harness that information to predict and test the routes that bactericides take when introduced. This will yield information about the routes bactericides travel when administered through delivery methods used by growers and the branch/petiole feeding technique. The researchers will develop two classes of bactericides, the first based on mining anti-CLas compounds naturally produced by microbes that inhabit Huanglongbing survivor trees in Florida, and the second based on silver and sulfur nanoparticles. Finally, the researchers will also undertake an extension and outreach program for citrus growers and non-commercial citrus growers (homeowners and hobbyists). They also will perform an economic cost-benefit analysis for adoption of these treatments in the commercial citrus industry. In addition to Roper, the following UC Riverside scientists are involved: James Borneman, Philippe Rolshausen, David Jassby, Georgios Vidalakis, and Haizhou Liu. And, the following researchers from other institutions are involved: Robert Turgeon (Cornell University); Katherine Maloney (Point Loma Nazarene University); Pieter Dorrestein (UC San Diego); Greg McCollum (U.S. Department of Agriculture), and Jonathan Kaplan (California State University, Sacramento). The grant is one of four given by the U.S. Department of Agriculture’s National Institute of Food and Agriculture (NIFA) to combat Huanglongbing. The funding is made possible through NIFA’s Specialty Crop Research Initiative Citrus Disease Research and Extension Program, authorized by the 2014 Farm Bill.